Hybrid enzyme catalysts synthesized by a de novo approach for expanding biocatalysis

The two major challenges in industrial enzymatic catalysis are the limited number of chemical reaction types that catalyzed by enzymes and instability under harsh conditions catalysis. Expanding enzyme to a larger substrate scope greater variety reactions tuning microenvironment surrounding molecules achieve high performance urgently needed. In this account, we focus on our efforts using de novo approach synthesis hybrid catalysts can address these structure–function relationship is discussed reveal principles designing catalysts. We hope account will promote further toward fundamental research wide applications designed for expanding biocatalysis.